Animal Behavior Laboratory Manual

FORMAT FOR LAB REPORTS

Introduction -- Methods and Results -- Discussion -- References

Common Mistakes

Lab Manual Table of Contents

General

Lab reports should follow the standard format for scientific papers.   Each report must include the following sections in this order:  

  • Introduction
  • Methods
  • Results
  • Discussion
  • References

Reports must not exceed four pages of text (typed or printed double-spaced with 12-point or larger font or hand-written clearly).   You will have to make your reports succinct and clear.

Introduction

State the biological question addressed by your project and provide some background about previous experiments and relevant theory.   Your textbook for Animal Behavior is a good source for background material.   Cite the textbook, if you include any information from it (see References, below).

More background is provided by articles listed at the end of each section of the lab manual.   These articles are in scientific journals available in the Chemistry/Biology Library (across South Road from the Bell Tower) or on the library's web site (see boxes below).

If you use these articles in preparing your reports, cite your sources (see References, below).

You can find articles with the library's E-Journal Finder.

In the box labeled "By Title", enter important words from the title of the journal that contains the article you are interested in.   Click on "Search", then select the correct journal, then the correct volume number, and the correct pages for the article.

Sometimes you can go straight to an article by entering its title and/or the first author's last name in a search box.   Try "Quick Article Search" on the right side of the E-Journal Finder page ... or the search box near the top of the home page for any particular journal.

You can also conduct your own literature search by using . . .
  • BIOSIS Previews (a database of scientific articles in all fields of biology),

  • JSTOR (a database of many articles in biological journals but usually not including the most recent volumes), or

  • PubMed (this database does not include all articles on organismal biology).

All of these databases are available in the library's E-Research Tools.

To find BIOSIS Previews, click on "Biology" in the list below "By Subject" on the E-Research Tools page, and then click on the link at the top of the next page.

When you enter words for a search, include both a general term and a specific term ... and try a couple of different combinations of words before selecting the most relevant articles to read.

Always cite any sources you use in your report (see References, below).

At the end of your Introduction, provide a general description of your project in one sentence.   For example,

"We compared the stimuli that evoke schooling behavior in two species of fish".  
In addition, state the specific question or hypothesis under investigation.   For example,
"We asked the question, Does each species tend to school with fish that resemble themselves?"  
Alternatively,
"Our null hypothesis stated that a species does not school more with a stimulus resembling itself than with one that does not".

Methods

Describe briefly the animal you worked with, the equipment used, the procedures followed, and the methods of collecting and analyzing data.   This description should allow someone else to duplicate your study.   Also a reader should be able to judge the adequacy of your methods and appreciate your ingenuity in addressing the question posed in the Introduction.   Always use the past tense to describe your Methods and Results.

Results

Use text and graphs to describe the data you collected.   Do not present raw data.   Instead summarize your data:   calculate averages, ranges, standard deviations (if you know how) and use graphs or tables.  

Graphs or tables are a good way to present data in a clearly understandable way, but you must always

  • label your axes
  • provide a title or caption for each graph or table
  • include at least a sentence in your text to explain the take-home message of the graph or table
Present the results of your statistical tests (see the section on
Simple Statistics).  

A reader of your Results section should be able to grasp the results of your project quickly and clearly.   In this section you should not include any interpretation of the results.

Discussion

Your interpretation of the results goes in this section.   A well-written Discussion includes these steps:
  1. an answer to the question raised in the Introduction (or a statement of whether or not the null hypothesis was supported)
  2. an evaluation of the adequacy of your methods (Would some change in methods make the results more conclusive?)
  3. a comparison of your results with those from similar studies (as described in the textbook or additional reading)
  4. an explanation of the mechanisms that might have produced the results (Why did your subjects do what they did?)
  5. suggestions for further experiments or investigations
The last two items are the meat of your report.   All science tries to understand how the world works.   In our case, we are trying to understand why animals behave the way they do.   What mechanism (or alternative mechanisms) could explain the behavior you observed?  

Remember to think about both

  • proximate (phyiological) mechanisms and
  • ultimate (evolutionary) mechanisms of behavior.  
A proximate explanation might state,
"The fish in our experiment must recognize the differences between horizontal and vertical stripes and somehow associate one of these patterns with approach and schooling behavior."
You might then continue with some ideas about how this association arises!  

An ultimate explanation might state,

"Natural selection might favor individuals that associate with members of their own species because predators could not focus on odd or conspicuous targets".
Often thinking about the mechanisms of any one result leads to additional questions that can only be answered by additional experiments.   So item (4) above usually leads naturally to item (5) above.

References

In your report, include a reference for any assertion of biological fact or theory that is not common sense or common knowledge.   To provide a reference for a statement, do not use footnotes.   Instead follow the standard practice in scientific papers of citing just the author and date of a reference in your text and then providing a list of complete references at the end.  

For example, in the text you might write:

"Wilson (1975) presents a scenario for the eventual unification of the behavioral sciences,"   or

"Honeybees can communicate the distance and direction to food even when olfactory cues are not available (Gould, 1976)."

In your References section, you would then include the following entries:
Gould, J.   1976.   The honey bee dance-language controversy.   Quarterly Review of Biology 51:   211-244.   (These numbers are the volume and the pages of the journal just named.)

Wilson, E. O.   1976.   Sociobiology:   the new synthesis.   Belknap Press, Cambridge, Mass.   (In this case the citation is a book; pages usually are not indicated except for a direct quotation.)

Common Mistakes in Lab Reports

(1) Failure to distinguish between the different sections of the report.   For instance, it is a mistake to include interpretation in the Results or to begin describing the procedures in the Introduction.

(2) Stating that the results prove a hypothesis.   In fact, scientific studies never definitely prove anything.   On the other hand, they can definitely exclude some hypotheses in specific cases.   It is a common mistake to think that scientists prove hypotheses, in the sense that a mathematician might prove a theorem.   Instead, careful scientific reports conclude that the results are consistent with one hypothesis (or more than one), so that this hypothesis can be tentatively accepted, pending further study.   Of course, support for a hypothesis becomes stronger as more and more experiments eliminate alternatives.

(3) Lack of any interpretation at all.   Data, or "facts", are meaningless in themselves.   For example, a statement that paradise fish display more frequently to other paradise fish than they do to their image in a mirror is not an interpretation; it simply states an observation.   An interpretation might explain that a real fish is a more effective stimulus because it provides not only visual releasers but also olfactory and tactile ones.   This interpretation proposes a possible mechanism that could produce the data.   In other words, you must consider why you obtained the results you did.

(4) Writing obscurely by using jargon or science-speak.   Instead, use simple sentences (subject-verb-object) and normal vocabulary.   Use a scientific term only when necessary for clarity or economy of expression -- and only when its meaning has been clearly defined in your report or in the lectures or labs for this course.  

Always avoid using

  • complicated words,
  • strings of nouns modifying other nouns, and
  • weak endings for sentences.  

You can find each of these three mistakes in the following example:

"Decremental responses   to   mirror image stimulation   were found."
In contrast, the following sentence uses simple, clear language:
"The responses of fish to their images in a mirror decreased steadily".
Although all scientists are powerfully attracted to jargon (perhaps because it makes us feel like we have joined a secret club!), in fact the best scientists explain their experiments in simple, clear language.   Not only is it easier for others, even other scientists, to understand what they have discovered, but it is also clear that the authors themselves understand what they have done!